London's Pulse: Medical Officer of Health reports 1848-1972

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London County Council 1899

[Report of the Medical Officer of Health for London County Council]

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TABLE 1—continued.

Experiment.Description of the samples of sewage.Total number of Bacteria in 1 c.c. of crude sewage.Number of spores of Bacteria in 1 c.c. of crude sewage.Number of Bacteria causing liquefaction of the gelatine inRemarks.
1.2.3.4.5.
12Barking crude sewage. Apl. '29, 1898.5,200,000 (one experiment with 0.00001 c.c. sew-age).120 (average of three expts. with 001,0-05, and 0-1 c.c. sewage).Surface gelatine plates containing 0.0000l and 0 000025 c.c. sewage liquefied by third day.Colonies counted third and fourth day.The liquefaction of the gelatine with 0.00005 and 0.000l c.c. sewage (column 2) was so rapid as to prevent the colonies being counted.
13Barking crude sewage. May 3, 1898.7,260,000(aver-age of two expts. with 0.0000l and 0.00005 c.c. sewage).3,020 (average of twoexpts. with 0.0l and 0"05 c.c. sewage).270,000 (average of two expts. with 0.0000l and 0000025 c.c. sewage).Colonies counted third, fourth, and sixth days. The liquefaction of the gelatine with 0.0001 c.c. sewage (column 2) was so rapid as to prevent the colonies being counted.The culture containing 0.l c.c. sewage (column 3) was liquefied by the second day.
14Barking crude sewage. May 4, 1898.3,630,000(aver-age of two expts. with 0.000l and 0.0000l c.c. sewage).450 (one expt. with 01 c.c. sewage).900,000 (one expt. with 000001 c.c. sewage).Spores of B. Enteritidis present in O'l, 0.0l and 0.001 c.c. sewage. Enteritidis absent in anaerobic milk-culture, containing 0.000l c.c.B. Coli Communis, 100,000 in 1 c.c. sewage.As regards spores: 10 of B. Megate-rium, 20 of B. Mesentericus, 10 of B. Mycoides, and 10 of B. Subtilis in 1 c.c. sewage.B. Fluorescens liquefaciens, 40,000 in 1 c.c. sewage.Gas-forming proteus, 100,000 in 1 c.c. sewage.
15Crossness crude sewage. May 4, 1898.3,160,000(aver-age of two expts. with 0.000l and 0.00001 c.c. sewage).380 (one expt. with 01 c.c. sewage).400,000 (one expt. with 0.0000l c.c. sewage).Spores of B. Enteritidis present in 0.l, 0.0l and 0'001 c.c. sewage. Enteritidis absent in anaerobic milk-culture containing 0.0000l c.c. sewage.B. Subtilis and B. Mesentericus, 20 spores of each in 1 c.c. sewage.B. Coli Communis, 200,000 in 1 c.c. sewage.Gas-forming proteus, 100,000 in 1 c.c. sewage.