[Report of the Medical Officer of Health for London County Council] page -

London County Council 1899

[Report of the Medical Officer of Health for London County Council]

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Table: TABLE 1— continued.

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TABLE 1— continued.

Experiment.	Description of the samples of sewage.	Total number of Bacteria in 1 c.c. of crude sewage.	Number of spores of Bacteria in 1 c.c. of crude sewage.	Number of Bacteria causing liquefaction of the gelatine in 1 c.c. of crude sewage.	Remarks.
Experiment.	1.	2.	3.	4.	5.
6	Barking crude sewage. Apl. 15, 1898.	513,333 (average of three experiments with 0.0000l, 0.00005, and 0.000l c.c. sewage).	263 (average of three expts. with 0 01, 0 05 and 01 c.c. sewage).	220,000 (average of three expts. with 0.0000l 0.000025 and 0 00005 c.c. sew-age).	The colonies were counted on the third and fourth days. The ratio between the liquefying bacteria and the total number of micro-organisms was 1 to 2.34.
7	Barking crude sewage. Apl. 20, 1898.	6,920,000 (average of 2 expts. with 0.0001 and and 0.00001 c.c. sewage).	290 (one expt. with 01 c.c. sewage).	The liquefying colonies were very numerous.	Spores of B. Enteritidis Sporogenes present in 1.0 and O'l c.c. sewage. B. Enteritidis not present in anaerobic milk-cultures containing 0.0001 and 0.001 c.c. sewage. 40 colonies indistinguishable from B. Coli in 0.000l c.c. sewage. No fluorescent colonies visible on the third day. Most of the colonies rapidly liquefying protean forms.
8	Crossness crude sewage. Apl. 20, 1898.	2,410,000 (one expt. with 0.000l c.c. sewage).	180 (one expt. with 0.1 c.c. sewage).	The liquefying colonies were very numerous.	Spores of B. Enteritidis Sporogenes present in 10 c.c. sewage. Enteri tidis not present in anaerobic milk-cultures containing 0.000l, 0.000l, 01, and O'l c.c. sewage. The milk, how. ever, had been prepared some time previous, which appears to inhibit the growth of Enteritidis, as pointed out by Dr. Klein. No fluorescent colonies visible on the third day. Most of the colonies rapidly liquefying protean forms. Thirteen colonies typical of B. Coli counted in a phenol-gelatine plate cultivation containing 0.000l c.c. of crude sewage.
9	Barking crude sewage. Apl. 22, 1898.	4,300,000 (average of two expts. with 0 00001 and 0.00005 c.c. sewage).	400 (one expt. with 001 c.c. sewage).	333,000 (average of three expts with 0.0000l, 0 000025,000005 c.c. sewage).	The colonies were counted on the third day. The liquefaction of the gelatine with 0.000l c.c. sewage (column 2) was so rapid that the colonies could not be counted on the third day. The liquefaction of the gelatine with 0.05 and 0.1 c.c. sewage (column 3) was so rapid that the colonies could not be counted on the third day.
10	Barking crude sewage. Apl. 27, 1898.	3,850,000 (average of two expts. with 0 0001 and 0.00001 c.c. sewage).	470 (one expt. with 0.1 c.c. sewage).	The liquefying colonies were very numerous.	Spores of B. Enteritidis present in 0.5 c. c. and O'l c.c. sewage. Enteritidis not present in anaerobic milk-culture containing 0.01 c.c. sewage. 10 spores of B. Mycoides, and 10 of B. Subtilis, in 1 c.c. sewage. B. Fluorescens liquefaciens, 10,000 in 1 c.c. sewage. No colonies visible of B. Fluorescens non-liquefaciens on the third day. 9 colonies indistinguishable from B. Coli Communis in surface phenol-gelatine plate containing 0.0000l c.c. sewage. Several of these were tested in "gelatine shake" cultures, and showed numerous bubbles the next day. Protean forms were very numerous.
11	Crossness crude sewage. Apl. 27, 1898.	3,300,000 (average of two expts. with 0.000l and 0.0000l c.c. crude sewage).	400 (one expt. with O'l c.c. sewage).	The liquefying colonies were very numerous.	Spores of B. Enteritidis present in 0.5, 0.1, and 0.01 c.c. sewage. B. Coli Communis, 200,000 in 1 c.c. sewage. On the third day no fluorescent colonies in 0.000l c.c. sewage. Colonies chiefly protean in character.