| 1 | Effluent from chemical precipitation works at Barking, Nov. 2, 1898. | 5,200,000 (two expts. with 0.0001 and 0.00001 c.c. effluent). | 100 (one expt. with 0.1 c.c. effluent). | 1,100,000 (one expt. with 0.0001 c.c. effluent). | Spores of B. enteritidis present in 0.1, 0.01 c.c. absent in 0.001 c.c.Five colonies indistinguishable from B. coli in phenol gelatine plate containing 0.00001 c.c. effluent (500,000 per c.c. of effluent).The plate, containing 0.0001 c.c. effluent (col. 2), was too crowded to allow of the colonies being accurately counted.The plate, containing 0.1 c.c. effluent (col. 3), contained some rapidly liquefying species, so that the numbers given are only approximate. |
| 2 | Effluent from chemical precipitation works at Crossness, Nov. 2,1898. | 9,800,000 (two expts. with 0.0001 and 0.00001 c.c. effluent). | 300 (one expt. with 0.1 c.c. effluent). | 1,000,000 (one expt. with 0.0001 c.c. effluent). | Spores of B. enteritidis present in 0.1 and 0.0l c.c.; absent in 0.001 c.c.Twenty-six colonies indistinguishable from B. coli in phenol gelatine plate containing 0.00001 c.c. effluent (2,600,000 per c.c. of effluent).The 0.00001 c.c. plate (col. 2) was counted, but not the 0.0001 c.c. plate, as this was too crowded. |
| 3 | Thames water collected off Greenhithe at about 2 p.m., October 12th, 1898, on a tide which had ebbed from Crossness for about two hours. The sample was, therefore, judged to be free from admixture with recent sewage effluent.(Dry weather.) | 10,000 | 63 | No record. | At least 1 but less than 10 spores of B. enteritidis per c.c. of water.No colonies of B. coli observed in phenol gelatine plate containing as small an amount of water as 0.001 c.c. That B. coli was present, however, in the water need not be doubted.There were present in the cultures numerous colonies of B. fluorescens liquefaciens and nonliquefaciens, B. mesentericus, B. mycoides, and proteus forms were also found. |
| 4 | Thames water, taken at low tide at Barking, and outside the influence of sewage discharge, as far as this was possible, Nov. 3, 1898.(Dry weather.) | 34,400 | 89 | (A Surfacegelatine plate culture containing 0.1 c.c. of the water was completely liquefied by the 2nd day). | At least 1 but less than 10 spores of B. enteritidis per c.c. of water.Eighty-four colonies, indistinguishable from B. coli, in phenol gelatine plate containing 0.1 c.c. of the water.There were present in the cultures, colonies of B. mesentericus, proteus zenkeri, B. fluorescens liquefaciens etc.Dr. Klein inoculated a guinea-pig with 1 c.c. of milk culture (containing 1.0 c.c. of water) which showed the typical enteritidis change. The animal died, and presented upon post-mortem examination the usual appearances; sanguineous exudation full of bacilli, etc. |
| 5 | Thames water, sample taken in mid-stream between Sunbury and Hampton,just above in take of the Southwark and Vauxhall Water Co. 10.40 a.m., Nov. 15, 1898 (dry weather). | 5,100 | 56 | No record. | Spores of B. enteritidis sporogenes absent in 1.0, 5.0, 10.0, 100.0 c.c.; present in 300 c.c. Dr. Klein inoculated a guinea-pig with 1 o.c. from latter culture. The animal died in less than 24 hours, and on post-mortem examination presented the usual appearances—swollen belly, sanguineous exudation swarming with bacilli, &c.Forty gas-forming B. coli per c.c. of water.Colonies of B. fluorescens liquefaciens and nonliquefaciens and proteus-forms present in the cultures. Also a large number of colonies of B. aquatilis sulcatus type.The presence of B. enteritidis in 300 c.c. of the water was demonstrated by a special filtration process. |
