| 1. | 2. | 3. | 4. | 5. |
| 6 | Crossness effluent from 4-foot cokebed, May 25, 1898. | 6,100,000 (one expt. with 0.00001 c.c. effluent.) | 380 (one expt. with 0.l c.c. effluent). | 700,000 (one expt. with 0.0000l c.c. effluent). | The liquefaction of the gelatine with 0.0001 c.c. effluent was so rapid that the culture could not be counted; the cultivation, however, containing 0.0000l o.c. effluent was counted.Spores of B. enteritidis present in 0.l, 0.0l and 0.001 c.c. effluent. Dr. Klein inoculated a guineapig with 1 c.c. of the whey from an anaerobic milk culture containing 0.00l c.c. effluent. The following day the animal was dead, and presented on examination the appearances which are typical of enteritidis (extensive gangrene, sanguineous exudation full of bacilli, etc.).Gas-forming coli, 700,000 per c.c. of effluent.No fluorescent colonies noted in 0.00001 c.c. effluent (5th day). |
| 7 | Crossness crude sewage, June 9,1898. | 6,500,000 (one expt. with 0.0000l c.c. sewage). | 560 (one expt. with 0.l c.c. sewage). | 1,400,000 (one expt. with 0.0000l c.c. sewage). | Spores of B. enteritidis present in 0.l and 0.0l c.c. sewage.In the cultivation for spores (0.l c.c. sewage, col. 3), several colonies of B. mesentericus.Gas-forming coli, 200,000 in 1 c.c. sewage.No fluorescent colonies noted in any of the cultures (4th day)."Sewage proteus," at least 100,000 per c.c. of sewage. |
| 8 | Crossness effluent from 4-foot cokebed, June 9 1898. | 1,200,000 (average of three expts. with 0.0000l effluent in each case). | 230 (one expt. with 0.l c.c. effluent). | 200,000 (one expt. with 0.0000l c.c. effluent). | Spores of B. enteritidis present in 0.l and 0.0l c.c. effluent.Gas-forming col., 100,000 in 1 c.c. effluent.No fluorescent colonies noted in any of the cultures (4th day)."Sewage proteus," 100,000 in 1 c.c. effluent.Anaerobic grape sugar gelatine cultivations were made with similar amounts of effluent (expt. 8) and crude sewage (expt. 7); the colonies were certainly more numerous in the culture made from the crude sewage. |
| 9 | Crossness crude sewage, June 15,1898. | 4,000,000 (one expt. with 0.00001 c.c. sewage). | 180 (one expt. with 0.l c.c. sewage). | 800,000 (one expt. with0.00001 c.c. sewage). | Spores of B. enteritidis present in 0.l c.c. sewage.In the cultivation for spores (0.l c.c. sewage, col. 3), 1 colony of B. mycoides.Gas-forming coli, 300,000 per c.c. sewage.B. jluorescens liquefaciens, 100,000 in 1 c.c. sewage.(a) 0.0001; (6) 0.000001; and (c) 0.0000001 c.c. sewage inoculated into each of three bouillon tubes and incubated at 20° C.; on 7th day no growth in (c), growth and offensive smell in (a) and (b)—(a) gave strong indol reaction and (6) faint trace. |
| 10 | Crossness effluent from 4-foot cokebed, June 15, 1898. | 5,300,000 (one expt. with 0.0000l c.c. effluent). | 300 (one expt. with 0.l c.c. effluent). | 1,100,000 (one expt. with 0.0000l c.c. effluent). | Spores of B. enteritidis present in 0.l c.c. effluent.Gas-forming coli, 600,000 per c.c. effluent, (a) 0.0001; (6) 0.000001; and (c) 0.0000001 c.c. effluent inoculated into each of three bouillon tubes and incubated at 20° C.; on 7th day no growth in (c), growth and offensive smell in (a) and (6)—(a) and (6) both gave distinct indol reaction. |
| 11 | Crossness crude sewage, June 22,1898. | 9,100,000 (one expt. with 0.00001 c.c. sewage). | 310 (one expt. with 0.l c.c. sewage). | 900,000 (one expt. with 0.0000l c.c. sewage). | Spores of B. enteritidis present in 0.l, 0.01 and 0.00l c.c. sewage.Gas-forming coli, 300,000 in 1 c.c. sewage. " Sewaqe proteus," 100,000 per c.c. sewage. |
| 12 | Crossness effluent from 4-foot cokebed, June 22, 1898. | 3,000,000 (one expt. with 0.0000l c.c. effluent.) | 60(one expt. with 0.l c.c. effluent). | 600,000 (one expt. 0.0000l c.c. effluent). | Spores of B. enteritidis present in 0.l c.c.; absent in 0.01 and 0.00l c.c. effluent.No colonies of "sewage proteus" in 0.00001 c.c. effluent.Gas-forming coli, 300,000 per c.c. effluent. B. Jluorescens liquefaciens, 100,000 in 1 c.c. effluent. |
