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5. Plate cultures (under a low power, Leitz, 3 objective, 1 ocular)—
(а) Agar (37° C.)—The large colonies are roughly circular' in shape and the smaller ones
irregular. Wavy granulation is well marked, and at the edge of the colonies separate
loops of cocci can be made out. The colonies are small and transparent-looking.*
(b) Gelatine (20° C.)—The colonies are very irregular in shape, and at the edge loops of
cocci may be made out. They are small and transparent, and show a coarse wavy
granulation.†
6. Streak cultures—
(а) Agar (37° C.)—The growth is composed of separate colonies which are transparent and
granular-looking, and are rather large for a streptococcus.
(b) Gelatine (20° C.)—By the 13th day the colonies have attained a fair size ; they are
irregular in shape and tend to run into each other. No liquefaction.
7. Gelatine at 37° C.—Resemble Streptococcus XII.
8. Broth cultures (37° C.)—The cloudiness is not well marked and is somewhat granular in character.
On shaking the tube gently viscous white masses with cirrhus-like extensions rise from the foot and float
throughout the liquid.
9. Litmus milk cultures (37° C.)—Slight acidity, but no clot (15th day).
10. Potato cultures (37° C.)—No visible growth (8th day).
1. Name—Streptococcus XIV.
2. Source—1/1000 c.c., Crossness crude sewage.
3. Date—December 15th, 1898.
4. Morphology—Stains well by Gram's method. The chains are short and tend to cohere in masses.
The cells are somewhat irregular in shape.
ft. Plate cultures (under a low power, Leitz, 3 objective, 1 ocular) —
(a) Agar (37° C.)—The colonies are small, nearly circular in shape, with a fairly firm edge.
They are granular and transparent-looking.
(b) Gelatine (20° C.)—The colonies are roughly circular in shape. Wavy granulation is
well marked. The edge may have a slightly ragged appearance, but no separate loops
of cocci can be made out. No liquefaction.§
6. Streak cultures—
(a) Agar (37° C.)—The colonies are separate, granular and transparent-looking, but are
of rather a large size for a streptococcus.
(b) Gelatine (20° C.)—Resembles Streptococcus XII. No liquefaction (13th day).
7. Gelatine at 37° C.—Resembles Streptococcus XII.
8. Broth cultures (37° C.)—The broth remains nearly transparent. At the foot of the tube the
growth collects as a viscous white mass.
9. Litmus milk cultures (37° C.)—Distinct acidity, but no clot (11th day).
1. Name—Streptococcus XV.
2. Source—1/1000 c. c., Crossness crude sewage.
3. Date—December 21st, 1898.
4. Morphology—Stains well by Gram's method. Chains of cocci are usually short, but some are of
medium length.
5. Plate cultures (under a low power, Leitz, 3 objective, 1 ocular)—
(a) Agar (37° C.)—Small, transparent, circular, granular colonies with firm edge.
(b) Gelatine (20° C.)—Small colonies of circular shape and with clean edge. They may
present a nucleated appearance. They are granular and transparent-looking. No
liquefaction.
6. Streak cultures—
(a) Agar (37° C.)—A barely visible white film, which on close observation is seen to be
made up of separate colonies (minute and transparent-looking).
(b) Gelatine (20° C.)—Resembles somewhat Streptococcus XII. No liquefaction (13th day).
7. Broth cultures (37° C.)—Diffuse cloudiness. At the foot of the tube a white viscous deposit,
which on shaking the tube rises in the form of a spiral.
8. Litmus milk cultures (37° C.)—In two days, distinct acid but no clot; 5th day, ditto; 11th
day, ditto.
9. Eemarks—1 c.c. of a 24 hours' broth culture (37° C.) injected subcutaneously into a mouse.
The mouse died on the 5th day. Microscopic preparations were made from the peritoneal fluid, &c.
Cocci were present, but also many putrefactive •bacilli. Agar plates made from the spleen were overgrown
with putrefactive organisms, and no streptococci could be isolated.
1. Name—Streptococcus XVI.
2. Source—1/1000 c.c., effluent from 4 ft. coke-bed at Crossness.
3. Date—December 21st, 1898.
4. Morphology—Stains well by Gram's method. Long chains of cocci matted together in masses.
5. Plate cultures (under a low power, Leitz, 3 objective, 1 ocular)—
(а) Agar (37° C.)—Minute transparent colonies, usually of circular shape, and showing wavy
granulation. No separate loops of cocci at periphery.
(b) Gelatine (20° C.)—Small, circular, transparent and granular-looking colonies. No
liquefaction.
6. Streak cultures—
(а) Agar (37° C.)—Thin, delicate film, which on close examination is seen to be made up of
minute separate colonies.
(b) Gelatine (20° C.)—Resembles Streptococcus X.
7. Broth cultures (37° C.)—Broth remains perfectly transparent. At foot of tube white conglomerate
mass.
8. Litmus milk cultures (37° C.)—Second day, no visible change; 5th day, slight acidity, but no
clot. Later, acidity more decided. No clot 11th day.
* Plate IV., fig. A (h).
† Plate IV., fig. A (i).
§ Plate IV., fig. A (j).
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