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1. Name—Streptococcus XVII.
2. Source—1/1000 c.c., effluent from 4 ft. coke-bed at Crossness.
3. Date—December 21st, 1898.
4. Morphology—Stains well by Gram's method. Chains of cocci short. The cells tend to cohere in
masses simulating a staphylococcus.
5. Plate cultures (under a low power, Leitz, 3 objective, 1 ocular)—
(а) Agar (37° C.)—Small, granular and transparent-looking colonies, nearly circular in
shape. The edge of the colonies has a ragged appearance, but no separate loops of cocci
can be made out.
(b) Gelatine (20° C.)—Minute colonies of circular shape, showing faint granulation. The
colonies are transparent-looking and some have a nucleated appearance. The edge is
firm and no separate loops of cocci are visible. No liquefaction.
6. Streak cultures—
(а) Agar (37° C.)—The growth presents no special characters worthy of note.
(b) Gelatine (20° C.)—Resembles somewhat Streptococcus XII. No liquefaction.
7. Broth cultures (37° C.)—Broth remains quite transparent. On sides of tube cirrhus-like, streaky,
white growth. At foot of tube, viscous white deposit.*
8. Litmus milk cultures (37° C.)—In two days, distinct acid, but no clot; 5th day, ditto; lltli
day, ditto.
1. Name—Streptococcus XVIII.
2. Source—1/1000 c.c., Crossness crude sewage.
3. Date—December 21st, 1898.
4. Morphology —Stains well by Gram's method. Short to medium chains.
5. Plate cultures (under a low power, Leitz, 3 objective, 1 ocular)—
(а) Agar (37° C.)—Colonies circular with firm edge; transparent and faintly granular.
(b) Gelatine (20° C.)—Most of the colonies seem to be made up of two portions, a granular,
irregularly-shaped, darker portion, and a more transparent and larger portion, which
is less granular. Some of the colonies, however, appeared to be composed of a collection
of delicate loops like S. Longus type. No liquefaction.†
6. Streak cultures—
(a) Agar (37° C.)—Resembles somewhat Streptococcus XV.
(b) Gelatine (20° C.)—On the 13th day the growth resembled Streptococcus XII., but in a
few places delicate filamentous extensions like fine seaweed may be seen. No liquefaction.
7. Broth cultures (37° C.)—Diffuse cloudiness, and at foot of tube viscous white deposit, which on
shaking the tube rises in the form of a spiral.
8. Litmus milk cultures—No visible change, even after 11 days' incubation.
1. Name—Streptococcus XIX.
2. Source—1/1000 c.c., effluent from the primary coarse bed (series A) at Barking.
3. Date—November 1st, 1899.
4. Morphology—Stains well by Gram's method. The chains of cocci are of medium length.§
5. Plate culiures (under a low power, Leitz, 3 objective, 1 ocular) —
(a) Agar (37° C.)—Irregularly-shaped, transparent colonies, showing well-marked wavy
granulation. At the edge of some of the colonies separate loops of cocci are clearly
visible.
(b) Gelatine (20° C.)—Small transparent colonies, showing faint wavy granulation. Usually
circular in shape with clean edge. No liquefaction.
6. Streak cultures—
(a) Agar (37° C.)—No record.
(b) Gelatine (20° C.)—Minute, transparent, circular colonies. No liquefaction.
7. Broth cultures (37° C.)—Some diffuse cloudiness, but granular cloudiness as well. On sides of
tube, streaky, cirrhus-like, white growth.
8. Litmus milk cultures (37° C.)—In two days strong acid, but no clot; 4th day, ditto.
9. Remarks—A mouse inoculated subcutaneously with 1 c.c. broth culture remained apparently
unaffected.
1. Name—Streptococcus XX.
2. Source—1/1000 c.c., effluent from the secondary coarse bed (series A) at Barking.
3. Date—November 1st, 1899.
4. Morphology—Stains well by Gram's method. Resembles Streptococcus XIX.¶
5. Plate cultures (under a low power, Leitz, 3 objective, 1 ocular)—
(a) Agar (37° C.)—Minute, transparent colonies, nearly circular in shape with clean edge.
Wavy granulation very faint. Some of the colonies are more granular-looking and have
a slightly frayed edge.
(b) Gelatine (20° C.)—Resembles Streptococcus XIX.
6. Streak cultures—
(a) Agar (37° C.)—No record.
(b) Gelatine (20° C.)—Very similar to Streptococcus XIX., but the colonies are more transparent-looking.
7. Broth cultures (37° C.)—Resembles Streptococcus XIX.
8. Litmus milk cultures—In two days, strong acid but no clot; 4th day, strong acid but no clot.
9. Remarks—A mouse inoculated subcutaneously with 1 c.c. of a broth culture was apparently
unaffected.
* Plate IV., fig. B (f).
§ Plate I., fig. 2.
†Plate IV., fig. A (k.
¶ late I., fig. 3.