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7. Broth cultures (37° C.)—Diffuse cloudiness; white deposit, at the foot of the tube; on shaking
the tube deposit rises in the form of a spiral.
8. Litmus milk cultures (37° C.)—Feeble acidity in two days. Later, distinct acidity, but no clot
(11th day).
9. Remarks—1 c.c. of a 24 hours' (at 37° C.) broth culture injected subcutaneously in a mouse. The
mouse died on the 5th day. Cocci present in juice of spleen and kidneys. From the spleen a pure culture
of a streptococcus was obtained, forming chains of medium length, and which differed somewhat from
the above. In broth the fluid remained clear, and at the foot of the tube the growth occurred as a white
conglomerate mass. In gelatine at 37° C. very slight diffuse cloudiness, and at the foot of tube a loose
flocculent cloudy growth. In gelatine plate cultures, colonies irregular in shape with uneven edge, wavy
granulation well marked.
1. Name—Streptococcus X.
2. Source—1/1000 c.c., effluent from 6 ft. secondary coke-bed at Crossness.
3. Date—December 15th, 1898.
4. Morphology—Stains well by Gram's method. Chains of cocci of medium length.*
5. Agar plate culture, 37° C. (under a low power, Leitz, 3 objective, 1 ocular)—Irregularly-shaped
colonies with broken up edge. Transparent with wavy granulation, and at edge loops of cocci can be
made out.t
6. Streak cultures—
(а) Agar (37° C.)—The growth is made up of numerous minute separate transparent colonies
which at first sight appears like a continuous filmy growth.
(b) Gelatine (20° C.)—By the 13th day colonies of varying size. They are very irregular in
shape, with lobed and fissured borders and delicate filamentous extensions like fine seaweed.
The colonies are white, but towards centre the colour is yellowish-white.
7. Broth cultures (37° C.)—Diffuse cloudiness; at foot of tube white viscous deposit which on
shaking the tube rises in spiral form.
8. Litmus milk cultures (37° C.)—In two days distinct acidity, but no clot; 11th day still no clot.
1. Name—Streptococcus XI.
2. Source—1/1000 c.c., Crossness crude sewage.
3. Date—December 15th, 1898.
4. Morphology—Stains well by Gram's method. Chains of cocci of variable length, but usually short.
5. Plate cultures (under a low power, Leitz, 3 objective, 1 ocular)—
(a) Agar (37° C.)—Most of the colonies minute, transparent and granular with firm edge.
(b) Gelatine (20° C.)—The majority of the colonies are nearly circular in shape : they are
transparent looking and faintly granular with firm edge. No liquefaction.§
6. Streak cultures—
(а) Agar (37° C.)—The growth at first sight appears like a continuous film, but is really
made up of separate minute transparent colonies.
(b) Gelatine (20° C.)—Small transparent faintly granular colonies. No liquefaction
(13th day).
7. Broth cultures (37° C.)—Diffuse cloudiness ; at the foot of the tube viscous white deposit, which
on shaking the tube rises in the form of a spiral.
8. Litmus milk cultures (37° C.)—No visible change, even after 11 days' incubation.
1. Name—Streptococcus XII.
2. Source—1/1000 c.c.. Crossness crude sewage.
3. Date—December 15th, 1898.
4. Morphology—Stains well by Gram's method. A streptococcus forming very short chains.
5. Plate cultures (under a low power, Leitz, 3 objective, 1 ocular) —
(а) Agar (37° C.)—Small circular colonies with firm edge, transparent and granular looking.
(b) Gelatine (20° C.)—The colonies are usually circular: they are faintly granular and
transparent looking. The edge is clean. No liquefaction.¶
6. Streak cultures—
(a) Agar (37° C.)—The colonies are rather large for a streptococcus; they have a granular
and transparent appearance.
(b) Gelatine (20° C.)—The mass of the growth appears as a continuous film ; but at the edge
and upper portion of the streak the colonies are separate and are small with a granular
transparent appearance. No liquefaction.
7. Gelatine at 37° C.—A granular white deposit forms at the foot of the tube, and the gelatine
throughout is full of minute granular specks.
8. Broth cultures (37° C.)—The broth is clear, but on sides of tube white cirrhus-like growth, and
at foot of tube viscous white conglomerate mass.
9. Litmus milk cultures (37° C.)—Slight acidity, but no clot (11th day).
1. Name—Streptococcus XIII.
2. Source—1/1000 c.c., Crossness crude sewage.
3. Date—December 15th, 1898.
4. Morpholoyy—Stains well by Gram's method. It was difficult to determine whether this microbe
was a streptococcus or a staphylococcus. Finally, it was classed as a streptococcus.
* Plate I., fig. 1. † Plate IV., fig. A (e).
§ Plate IV., fig. A (f).
¶ Plate IV., fig. A (g).