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5. Plate cultures (under a low power, Leitz, 3 objective, 1 ocular)—
(a) Agar (37° C.)—Appearances very similar to Streptococcus III., i.e., some colonies circular
and granular with clean edges; others more or less irregular in shape with ragged edges.
Apparently, however, fewer colonies of irregular shape and ragged edges than in the
cases of Streptococci III. and IV.
(b) Gelatine (20° C.)—As there was practically no growth in gelatine streak cultures, no
gelatine plate cultures were made.
6. Streak cultures—
(а) Agar (37° C.)—Growth somewhat similar in appearance to Streptococcus III.
(b) Gelatine (20° C.)—Practically no growth (13th day).
7. Gelatine at 37° C.—Appearance very like Streptococcus III.
8. Broth cultures (37° C.)—Diffuse cloudiness.
9. Litmus milk cultures—Distinct acidity, but no clot (11th day).
1. Name—Streptococcus VI.
2. Source—1/1000 c.c., effluent from 4 ft. coke-bed at Crossness.
3. Date—November 29th, 1898.
4. Morphology—Stains well by Gram's method. Chains of cocci of variable length, but usually short.
5. Plate cultures (under a low power, Leitz, 3 objective, 1 ocular)—
(a) Agar (37° C.)—Small transparent granular colonies, usually circular in shape. Most of
the colonies have a firm edge, but in some of them the edge has a ragged appearance.
(b) Gelatine (20° C.)—No record.
6. Streak cultures—
(а) Agar (37° C.)—The growth resembles Streptococcus I.
(b) Gelatine (20° C.)—By the 13th day the colonies have attained a fair size: they are
granular, and at the edge have a lobed and fissured appearance. The colonies are white,
but towards the centre they are yellowish-white in colour. No liquefaction.
7. Broth cultures (37° C.)—Diffuse cloudiness; at the foot of the tube white viscous growth, which
on shaking the tube rises in the form of a spiral.
8. Litmus milk cultures (37° C.)—Very slight acidity, no clot (10th day).
1. Name—Streptococcus VII.
2. Source—1/1000 c.c., effluent from 4 ft. coke-bed at Crossness.
3. Bate—November 29th, 1898.
4. Morphology—Stains well by Gram's method. Chains of cocci of variable length, but usually short.
5. Plate cultures (under a low power, Leitz, 3 objective, 1 ocular)—
(a) Agar (37° C.)—Small more or less circular colonies, transparent and granular. Most of
the colonies have a firm edge, but in others the edge is broken up and uneven in
character.
(b) Gelatine (20° C.)—As there was practically no growth in gelatine streak cultures (13th
day at 20° C.), no gelatine plate cultures were made.
6. Streak cultures—
(a) Agar (37° C.)—No record.
(b) Gelatine (20° C.)—Practically no growth (13th day at 20° C.).
7. Broth cultures (37° C.)—Some diffuse cloudiness, and at the foot of the tube granular masses
as in Streptococcus I.
8. Litmus milk cultures (37° C.)—Slight acidity, but no clot (10th day).
1. Name—Streptococcus VIII.
2. Source—1/1000 c.c., effluent from 6 ft. primary coke-bed at Crossness.
3. Date—December 9th, 1898.
4. Morphology—Stains well by Gram's method. Chains of cocci of variable length.
5. Agar plate cultures (37° C.) (under a low power, Leitz, 3 objective, 1 ocular)—Small transparent
granular colonies with firm edges and roughly circular in shape.
6. Streak cultures—
(a) Agar (37° C.)—The growth appears as a thin transparent greyish-white film, which on
close examination is seen to be made up of minute separate colonies.
(b) Gelatine (20° C.)—Colonies are transparent and granular-looking. No liquefaction
(13th day).
7. Broth cultures (37° C.)—Diffuse cloudiness; at the foot of the tube a white deposit, which rises
in the form of a spiral on shaking the tube.
8. Litmus milk cultures (37° C.)—Strong acidity, but no clot (11th day).
9. Remarks—1 c.c. of broth culture injected subcutaneously into a guinea-pig. The animal
remained apparently unaffected.
1. Name—Streptococcus IX.
2. Source—1/1000 c.c., effluent from 6 ft. secondary coke-bed at Crossness.
3. Date—December 15th, 1898.
4. Morphology—Stains well by Gram's method. Chains of cooci usually short, but some of medium
length.
5. Agar plate cultures, 37° C. (under a low power, Leitz, 3 objective, 1 ocular)—The majority of the
colonies are circular in shape with firm edges. They are small, granular and transparent looking.
6. Streak cultures—
(а) Agar (37° C.)—The growth at first sight looks like a continuous white film, but on close
inspection it is seen to be made up of numerous separate minute transparent colonies.
(b) Gelatine (20° C.)—Minute transparent colonies, more or less circular in shape. No
liquefaction.