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5. Plate cultures (under a low power, Leitz, 3 objective, 1 ocular)—
(а) Agar (37° C.)—Small transparent circular faintly-granular colonies with firm edges.
No loops of cocci are visible at the periphery.*
(b) Gelatine (20° C.)—Small transparent faintly-granular colonies, circular in shape with
clean edges. Old colonies may present a nucleated appearance. No appearance of
separate loops of cocci at the periphery of the colonies. No liquefaction.†
6. Streak cultures—
(а) Agar (37° C.)—Minute transparent colonies. The colonies are situated so close together
as to appear at first sight like a continuous film.
(b) Gelatine (20° C.)—Almost no growth, even after 13 days.
7. Gelatine at 37° C.—Granular cloudiness, with a white granular deposit at the foot of the tube.†
8. Broth cultures (37° C.)—Diffuse cloudiness; at the foot of the tube white viscous growth,
which on shaking the tube, rises in the form of a spiral.§
9. Litmus milk cultures (37° C.)—In 2 day's acidity ; in 4 days, strong acidity but no clot; 7th day,
still no clot; 25th day, still no clot.
10. Remarks—A mouse inoculated subcutaneously with 1 c.c. of a 4 days' broth culture died on the
7th day. Microscopic preparations made from the kidney juice showed tho presence of cocci, sometimes
arranged as diplo-cocci, and showing an appearance as of a surrounding capsule. This was not very distinct,
however. Agar plate cultures were made from the spleen, and a streptococcus was isolated, which differed
somewhat from the one above described. It produced diffuse cloudiness in broth and diffuse granular
cloudiness in gelatine at 37° C. Milk was rendered strongly acid and clotted.
1. Name—Streptococcus III.
2. Source—1/1000 c.c, Crossness crude sewage.
3. Date—November 29th, 1898.
4. Morphology—Stains well by Gram's method. Chains of cocci of variable length; most of them
are short.
5. Plate cultures (under a low power, Leitz, 3 objective, 1 ocular)—
(а) Agar (37° C.)—Minute transparent faintly-granular colonies. Most of the colonies are
circular with a clean edge. Some are irregular in shape with a ragged edge resembling
somewhat Streptococcus I.¶
(b) Gelatine (20° C.)—Small transparent colonies, roughly circular in shape with firm edges.
The colonies are faintly granular, and show no separate loops of cocci at the periphery.
No liquefaction.
6. Streak cultures —
(a) Agar (37° C.)—The growth appears at first sight like a continuous delicate film, but is
in reality composed of numerous minute transparent and separate colonies.
(b) Gelatine (20° C.)—Colonies are somewhat large for a streptococcus : they are roughly
circular in shape, and have a granular appearance. No liquefaction.
7. Gelatine at 37° C.—Granular cloudiness at the foot of the tube. Throughout the medium
granular cloudiness and slight diffuse cloudiness as well.
8. Broth cultures (37° C.)—Diffuse cloudiness throughout the medium.
9. Litmus milk cultures—Acidity, but no clot in 24 hours. Later, strong acid but no clot (14th day).
10. Remarks—1 c.c. of a 2 days' broth culture injected subcutaneously in a mouse produced no
apparent effect.
1. Name—Streptococcus TV.
2. Source—1/1000 c.c., effluent from 4 ft. coke-bed at Crossness.
3. Date—November 29th, 1898.
4. Morphology—Stains by Gram's method. Chains of cocci of variable length ; usually short,
however.
5. Plate cultures (under a low power, Leitz, 3 objective, 1 ocular)—
(a) Agar (37° C.)—Colonies very similar to Streptococcus III.
(b) Gelatine (20° C.)—As practically no growth occurred in gelatine streak cultures, no
gelatine plates were made.
6. Streak cultures—
(a) Agar (37° C.)—Growth resembles both Streptococcus I. and Streptococcus III.
(b) Gelatine (20° C.) —Practically no growth, even after 13 days.
7. Gelatine at 37° C.—Granular deposit at foot of tube and granular cloudiness throughout the
medium, but no diffuse cloudiness.||
8. Broth cultures (37° C.)—Diffuse cloudiness. Deposit at the foot of the tube coherent in masses
unlike Streptococcus III.
9. Litmus milk cultures (37° C.)—Distinct acidity, but no clot (11th day).
10. Remarks—1 c.c. of a 2 days' broth culture injected subcutaneously in a mouse. The mouse
died on 9th day. Cover glass specimens made from kidney juice, peritoneal fluid, &c., showed the
presence of cocci and diplococci (appearance suggestive of surrounding capsule, but not very distinct).
Agar plate cultures were made from the organs, but the plates were crowded with putrefaotive bacteria,
and no streptooocci were isolated.
1. Name—Streptococcus V.
2. Source—1/1000 c.c., effluent from 4 ft. coke-bed at Crossness.
3. Date—November 29th, 1898.
4. Morphology—Stains by Gram's method. Short chains and chains of medium length.
* Plate IV., fig. A (b). † Plate IV., fig. A (c). J Plate IV., fig. B (c). § Plate IV., fig. B (d).
¶ Plate IV., fig. A (d). || Plate IV., fig. B (e)