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With recent Agar culture of all three microbes, a, b and c injected guinea pigs,
two for each species, using considerable amounts of the cultures. The result was
nil, the animals remained well.
Series II.—With pieces of the Cheese mixed with a little moistened bread the
following animals were fed on two successive days; the amount of cheese eaten by
the animals was considerable:—
1. Four mice.
2. Three white rats.
3. Two guinea pigs.
4. Two rabbits.
The day after the first feeding all these animals were found perfectly well; the
day after the second feeding one rat was found dead.
This animal, on post-mortem, showed no change in the internal organs; the viscera
were not injected, the spleen and liver and lungs appeared normal, the intestines not
injected, but containing much gas.
Cultivations were made from the blood of the heart and of the spleen. These
cultures remained quite sterile.
Of the four mice that were fed with the Cheese one appeared ill two days after
the feeding; it was found dead four days after.
On post-mortem examination the intestines appeared slightly injected, but the
liver, spleen, kidney and lungs appeared normal.
Cultivations were made by rubbing a large lump of blood clot of the heart over
the surface of gelatine; the result was that five colonies of bacillus coli were
obtained. All other animals fed remained well and normal till this day,
December 3rd.
Series III.—With the turbid, milky, salt emulsion of the cheese injected
subcutaneously with considerable amounts:—
1. Three mice.
2. Three rats.
3. Two guinea pigs.
4. Two rabbits.
The result was nil; none of the animals showed any illness, local or general.
E. KLEIN.
CROYDON:—
A further outbreak affecting one family only has been since reported by
Dr. Richards, Medical Officer of Health for the County Borough of Croydon.
This Cheese was bought by a local retailer from a City firm, F & G, who obtained
it from the same shippers about the same date as all the previous consignments.